Journal: Cell Reports Methods
Article Title: Non-invasive real-time pulsed Doppler assessment of blood flow in mouse ophthalmic artery
doi: 10.1016/j.crmeth.2025.100983
Figure Lengend Snippet: Setup of non-invasive Doppler OphA blood flow velocity measurements at mouse orbital opening An anesthetized mouse is placed in a prone position on the ECG board. The Doppler probe sensor tip is at the temporal canthus (left and center). Doppler in-phase (I), quadrature (Q), and ECG signals are acquired, processed, displayed, and recorded in real-time (middle). Doppler velocity signal waveforms with major parameters are acquired for analysis (right). DVFS, Doppler flow velocity system; US, ultrasound; ECG, electrocardiogram; FV, flow velocity.
Article Snippet: The principle of operation of the DFVS is described in great detail elsewhere., , Mice were anesthetized with 2.0% isoflurane in the induction chamber and then transferred to a heated electrocardiogram (ECG) board (Mouse Monitor S, Indus Instruments, Webster, TX).
Techniques: